Tag
pilA1 SS

Part:BBa_K125300:Design

Designed by: Krystle Salazar and Grace Kwan   Group: iGEM08_Hawaii   (2008-07-25)

pilA1 signal sequence from cyanobacterium Synechocystis; secretes protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pilA promoter was synthesized as 4 different oligonucleotides that were annealed, then ligated together.

Primer name Primer Length G/C content Tm
pilA1_forward CTAGATGGCTAGTAATTTTAAATTCAAACTCCTCTCTCAAC 41 34.1% 65.3C
pilA1_reverse AGAGGAGTTTGAATTTAAAATTACTAGCCAT 31 29.0% 60.6C
pilA2_forward TCTCCAAAAAACGGGCAGAAGGTGGTACTAGTAGCGGCCGCTGCA 45 55.6% 76.8C
pilA2_reverse GCGGCCGCTACTAGTACCACCTTCTGCCCGTTTTTTGGAGAGTTGAG 47 55.3% 75.5C

Source

The pilA signal sequence is found at the amino terminal of pilA polypeptides synthesized by Synechocystis sp. PCC6803. The sequence here is that identified by Sergeyeko and Los.


References

Sergeyenko, Tatiana V and Los, Dmitry A. "Cyanobacterial leader peptides for protein secretion". FEMS Microbiology Letters 2006 (218): 351-357.